Small 125mm led Traffic Light simulation teaching traffic light characteristics of decorative traffic lights 125mm traffic light,125mm mini traffic light,125mm led traffic signal light Shenzhen Wide Way Optoelectronics Co., Ltd. , https://www.wdmtrafficlight.com
1. The 125-type traffic light adopts the C125MM lamp tube that our company has independently opened the mold, with a moderate size and a unique shape.
2. The 125 type traffic light adopts Taiwan Epistar chip lamp beads, which is bright, eye-catching and distinct in color.
3. The bottom of the 125 type traffic light adopts a waterproof connector for outlet, which has a good waterproof effect.
4. The 125 type traffic light adopts bracket installation, which is simple to operate and easy to install.
Install small LED traffic lights, simulate teaching traffic lights, and pay attention to when decorating traffic lights:
1. At an intersection with a guide lane, drive into the guide lane according to the required direction of travel;
2. Those who are ready to enter the roundabout let motor vehicles that are already in the intersection go ahead;
3. When turning to the left, turn to the left of the center of the intersection. Turn on the turn signal when turning, and turn on the low beam when driving at night;
4. When meeting the release signal, pass in turn;
5. When the stop signal is encountered, stop outside the stop line one by one. If there is no stop line, stop outside the intersection;
6. When turning right when there is a car in the same lane waiting for the release signal, stop and wait one by one;
7. At intersections with no direction indicator lights, turning motor vehicles let straight vehicles and pedestrians go first. Right-turning motor vehicles traveling in the opposite direction let left-turning vehicles go first.
Rat adenosine deaminase (ADA) ELISA kit instructions
Shanghai Xitang Biotechnology Co., Ltd. 021-55229872, 65333639
Rat adenosine deaminase (ADA) ELISA kit
 ( used in serum, plasma, cell culture supernatants and other biological fluids )
principle
This experiment used double antibody sandwich ABC-ELISA. The anti-rat ADA monoclonal antibody was coated on the microtiter plate, the standard and the ADA in the sample were combined with the monoclonal antibody, and biotinylated anti-rat ADA was added to form an immune complex attached to the plate, horseradish peroxidation. The enzyme-labeled Streptavidin is combined with biotin, and the substrate working solution is blue. Finally, the stop solution sulfuric acid is added, and the OD value is measured at 450 nm. The ADA concentration is directly proportional to the OD value, and the ADA in the sample can be obtained by drawing a standard curve. concentration.
Kit composition ( 2-8 ° C preservation)
Coated Wells
96 holes
Enzyme Conjugate
12ml
10× specimen dilution (Sample Buffer)
12ml
20×Wash Buffer
50ml
Standards: 2U / bottle
2 bottles
Substrate working fluid (TMB Solution)
12ml
Primary antibody working solution (Biotinylated Antibody)
12ml
Stop Solution
12ml
Prepare reagents and collect blood samples
1. Collect specimens: serum, plasma (EDTA), cell culture supernatant, tissue homogenate, etc., as soon as possible, store at 2-8 ° C for 48 hours; longer time must be frozen (-20 °C or -70 °C) Save to avoid repeated freezing and thawing.
2. Standard solution preparation: Add 1ml of distilled water before use and mix well to form 2000U/L solution. Set the standard tube 8 tube, the first tube plus the standard dilution 900ul, the second to the eighth tube to add the sample dilution 500ul. Add 100 ul of the 2000 U/L standard solution to the first tube, mix it, and then aspirate 500 ul with the sampler and transfer to the second tube. Repeat the dilution as described above, and remove 500 ul from the seventh tube and discard it. The eighth tube is a blank control.
3. The 10× specimen dilution was diluted 1:10 with distilled water (example: 1 ml concentrated dilution + 9 ml distilled water).
4. Washing solution: diluted 1:20 with distilled water (example: 1 ml concentrated washing solution added to 19 ml of distilled water)
Test procedure
1. Loading: Add 100 ul of standard or sample to be tested in each well. Mix the reaction plate thoroughly and let it stand at 37 °C for 120 minutes.
2. Wash the plate: Wash the plate thoroughly with washing solution 4-6 times, and dry it on the filter paper.
3. Add 100 ul of the first antibody working solution to each well. The reaction plate was thoroughly mixed and placed at 37 ° C for 60 minutes.
4. Wash the board: the same as before.
5. Add 100 ul of enzyme-labeled antibody working solution per well. The reaction plate was placed at 37 ° C for 30 minutes.
6. Wash the board: same as before.
7. Add 100 ul of substrate working solution per well, set 37 The reaction was carried out in the dark at °C for 15 minutes.
8. Add 100 ul of stop solution to each well and mix.
9. Measure the absorbance at 450 nm using a microplate reader within 30 minutes.
Result calculation and judgment
1. All OD values ​​should be subtracted from the blank value before calculation.
2. Using standard products 200, 100, 50, 25, 12.5, 6.25, 3.12, 0 U/L as the abscissa and OD as the ordinate, plot on the coordinate paper and draw the standard curve.
3. Find the corresponding ADA content on the graph based on the sample OD value.
Kit performance
1. Sensitivity: The minimum ADA detection concentration is less than 2U/L.
2. Specificity: Recombinant or natural rat ADA can be detected simultaneously. Does not cross-react with other cytokines in rats.
3. Repeatability: The coefficient of variation in both the plate and the plate is less than 10%.
Precautions
1. It is recommended to make double holes for the above standard holes and samples to be tested. The standard curve should be made at the same time for each measurement.
2. The washing process is critical. Insufficient washing will result in an accuracy error and an erroneous rise in the OD value.
3. After the slats are opened, the remaining slats should be sealed again to keep the slats dry .
4. This kit should be stored in a 4 o C refrigerator.
4. This kit is for scientific research only and cannot be used for clinical diagnosis!
Shanghai Xitang Biotechnology Co., Ltd. 021-55229872, 65333639
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