SunFire Columns: Waters' Best Silicone Matrix Universal Columns for Better Tooling for Food Safety Testing

SunFire Columns: Waters' Best Silicone Matrix Universal Columns for Better Tooling for Food Safety Testing

Waters' SunFire C 18 and C8 columns set a new benchmark for performance in the industry's silica-based reversed-phase C 18 and C8 columns, and Waters Corporation's research on filler particle synthesis and bond closure for many years And in the development of column products, the outstanding performance of the SunFire column. These properties are perfectly in line with the characteristics and needs of today's food safety testing technology.

● Universally excellent peak shape <br> Universal -low pH conditions generally have excellent peak shape for various compounds, suitable for multi-component residue detection

● High-capacity design <br> Especially suitable for trace component analysis, withstand high injection volume and is not prone to overload problems

● Excellent efficiency and resolution <br>Specially beneficial for food safety testing of sample matrices, including multi-component residue detection

● Multiple particle size and column size <br>2.5, 3.5, 5μm, column inner diameter range 1.0-4.6mm, column length 20-250mm, suitable for various analysis needs. The narrow internal diameter allows direct adaptation to the MS detector without shunting. Small particle size and short column lengths help chromatographers achieve higher sensitivity and higher throughput. The method transfer is easy and free between different column sizes.

● Excellent mass spectrometry compatibility <br>Because of its excellent particle synthesis technology and bonding/capping technology, good peak shape for alkaline analytes can be obtained even with low ionic strength conditions (such as 0.1% formic acid). It is not prone to shark fin-like overload peaks, ensuring resolution and sensitivity, which is especially suitable for food safety testing based on LCMS detection platform.

Its excellent stability under low pH conditions ensures that the background noise of the bonded phase is lost when using LCMS technology, as well as a more stable and durable column life.

Detection of multi-component residues of fungicides <Benzimidazoles, such as Thiabendazole, are fungicides conventionally used to protect fruits and vegetables. However, liquid phase analysis of these materials is often cumbersome. For example, bismuth, on most reversed-phase silica gel columns, shows significant tailing, especially when the analysis is carried out under acidic pH conditions.涕 灵 和 and Carbendazim have good retention and peak shape on Waters hybrid particle technology columns such as XTerra ® MS C 18 column at pH 10; however, high pH conditions are not suitable for other Simultaneous detection of a variety of fungicide components, for example, Thiophanate and Thiophanate Methylate, which are carbamate fungicides that are unstable in high pH mobile phases, such as When using high pH conditions for detection, it will be missed or the concentration will be inaccurate.

Excellent retention and peak shape for all of these fungicide analytes can be obtained using a SunFireTM C18 column at low pH conditions such as pH 3.7. It can be seen that the equimolar time-sharing of carbendazim and carbendazim using pH 3.7 has a tailing factor of only 1.2 at 10% peak height, which can be obtained with XTerra® columns at high pH. The peak shape is comparable. The result is that other silica C 18 columns are difficult to match under similar conditions (low pH).

Test Conditions

SunFireTM C18: 2.1x100mm, 3.5um, PN 186002534
Mobile phase A: Water mobile phase B: Acetonitrile mobile phase C: 500 mM ammonium formate buffer (pH 3.7)
Gradient or isocratic conditions as shown in the specs

Column temperature: 30 ° C
Instrument: Alliance 2695, Waters ZQ MS

Mass spectrometry conditions: cone voltage 25V, ESI+ mode (source temperature 120 ° C, desolvation temperature 350 ° C)

SPE condition
3cc Oasis MCX cartridge activation and balance: 1mL methanol rinse, 1mL water balance loading: sample solution with formic acid adjusted to pH3, sample loading at 5mL / min speed: 1mL 20:89:1 methanol / water / concentrated ammonia water wash Dissociation: 2mL 2% ammonia methanol

Since the carbamate is unstable in the alkali solution, the eluate is evaporated and dissolved in the mobile phase.

Gradient analysis of 6 fungicides was performed using a SunFireTM C18 column (50 ng/mL mix, 10 uL injection). Gradient conditions: 90:5:5 (A/B/C) in 10 min to 0:95:5 (A/B/C).

Analytical gradients of carbendazim and carbendazim (1 μg/mL mix, 10 μL injection) were performed using a SunFireTM C18 column. Elution conditions: 75:20:5 (A/B/C).

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