New method can not decipher gene epigenetic code

Researchers at the University of Pennsylvania have developed a new method for deciphering the DNA epigenetic code, using DNA deaminase for gene sequencing. They published a paper in the journal Nature Biotechnology on the 8th that the new sequencing method overcomes the limitations of bisulfite sequencing that has been used for decades and will help to understand more complex biological processes such as tumorigenesis.

Epigenetics refers to heritable changes in gene expression without alteration of the nucleotide sequence of the gene. The analysis of epigenetic code helps to better understand and diagnose a series of diseases including cancer, and thus becomes an important direction of medical research.

In the past few decades, the main method used to decipher the epigenetic code relied on a chemical called bisulfite. However, the sequencing method based on this substance is useful, but it has many limitations. The most important point is that bisulfite will destroy most of the DNA it contacts, and the sequencing material that can be left after modification will Very few.

This time, the new method developed by the University of Pennsylvania effectively overcomes these limitations. The researchers introduced a deaminase called the apolipoprotein messenger RNA editing enzyme-catalyzed polypeptide (APOBEC), which can effectively distinguish the cytosine modification state, and its effect can be achieved with the use of sulfurous acid. Hydrogen salts are similar but do not damage DNA.

Studies have shown that using a new method to determine the epigenetic code of a neuron requires much less DNA input than using bisulfite sequencing, only one-thousandth of the latter. In the past, some epigenomic studies were difficult to carry out due to sample scarcity, and the DNA input required for sequencing was greatly reduced, which means that the expansion of sequencing applications will help promote such research.

The researchers say that the new method overcomes many of the limitations of bisulfite-based sequencing methods, expands the scope of epigenetic analysis, and provides new tools for studying epigenetic codes, which provide a deeper understanding of neurodevelopment. Complex biological processes such as tumor formation are of great significance. (Liu Haiying)

Source: Technology Daily

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